Monday, August 19, 2019
Review Of Wwf Wrestling :: essays research papers
WWF: A Review of Professional Wrestlingà à à à à Bang! Fireworks as if it were the fourth of July. Thousands of people in an arena and billions watching around the world anxiously awaiting the arrival of their favorite superstar. Suddenly the crowd erupts as a man comes strutting down to the squared circle. Accompanied by a beautiful busty young woman, the wrestler is cheered by his loyal fans and booed by his enemies. Soon , another man enters the stadium the same way as the first man. The two men stip into the ring with a certain sense of cockiness about themselves. Before the referee can even tell everyone that the match has started, the two men are already battling in the “squared circle';. During the fight there are many suspenseful moments. Brutally beating each other for five minutes the match is finally over with some bloodshed. Today people are taking wwf professional wrestling to the extreme. WWF wrestling is one of the most watched shows on television today. WWF stands for the World Wrestling Federation. Wrestling starte d sometime it the mid to late sixties and has changed dramatically over the years. When wrestling first came to be the wrestlers weren’t like they were today. Back in the olden days of WWF wrestling, the storyline of wrestling wasn’t as vulgar, and the industry was not as wide spread throughout the world. Today the whole industry of the WWF is benefitting from merchandise being sold all over the globe. Even though many people may consider wrestling to be fake, know that some aspects are very real. There have been some instances where people have gotten hurt and even died from a stunt gone bad. There was an instance about half a year ago when a professional wrestler named Owen Hart was repelling from the rafters on a cable and the piece that was attached to his harness snapped and he plummeted 40 feet broke his neck and died. This was a tragic loss to every wrestler and to the whole WWF world. If this isn’t real you wouldn’t know what was. Another example of r eality is when Stone Cold Steve Austin was being suplexed (a professional wrestling move when your opponent lifts you in the air and slams you into the mat), his opponent landed Austin wrong on the mat. This resulted in a career ending surgery for Steve Austin. One thing that wrestling has done over the past couple of years is raised media uproar pertaining to violence at home between children.
Sunday, August 18, 2019
Indian Nationalism :: Asian Asian History
Indian Nationalism Factors Promoting Nationalism Racial arrogance - on the part of the British created resentment by Indians. They were treated as second-class citizens and were given only the poorest jobs. British in positions of power, such as General Mayo (Viceroy of India) openly made statements of racial superiority. Educated Indian professionals - felt they were denied equal opportunities within the "machinery" of British rule (such as the Indian Civil Service). They founded a nationalist movement that initially sought equal status for Indians and eventually sought full Indian independence. Anti-Westernisation - Britain had changed India's traditional institutions with apparently little respect for the existing culture and religion. British modernisation - probably helped spread the nationalist message. They had improved transport and communications. Indian Association 1885 - Began as a powerless "talking shop" but turned into the Indian National Congress, where Indians could voice their complaints against the British. Educated politicians used this parliament to rally support for the movement. Lord Curzon (Viceroy 1898-1905) gave no concessions to the natives. He allowed them no representation and saw them as inferior. Division of Bengal 1906 - was unpopular because it divided the regional cultures. In India, many people had greater affinity to their region than to the whole country. Splitting Bengal led to a swell of nationalist support. Factors Restraining Nationalism India was divided regionally - There was great cultural and religious divide across the regions. There were 200 different languages spoken across India so communication on a large scale was difficult. There were also many religions (including Sikhs, Muslims, and Hindus) who would not co-operate with each other. Indian Congress was divided - Liberal wing led by Gokhale, and extremist wing led by Tilak.
Saturday, August 17, 2019
Ted Bundy & terror
Ted Bundy was a terror for girls and women in 1970ââ¬â¢s. He was a serial killer and a rapist who is believed to have ruthlessly murdered more than 20 girls from 1974-1979. Theodore Robert Cowell was born on November 24, 1946 to Eleanor Louise Cowell in a Vermont residential home for unwed mothers.His grandparents were introduced to him as his parents and his mother as his elder sister since she was unmarried. ââ¬Å"Some, perhaps searching for some cause to Bundy's future actions, feel that Bundy's grandfather, Sam, may actually have fathered Ted out of an incestious relationship with Eleanor.The resulting confusion was the only known possbile truama in the young boy's lifeâ⬠(ââ¬Å"Predator; Ted Bundyâ⬠BEGINNINGS). Eleanor married Johnnie Bundy on May 19, 1951 and Theodore Robert Cowell adopted Bundy as his last name. Bundy did not have any unpleasant experience in his childhood (ââ¬Å"Predator; Ted Bundyâ⬠BEGINNINGS). By the time of his graduation Bundy had b ecome a stealer. He met his love Stephanie Brooks during his studies at the University of Washington. Even though love blossomed in their relationship it was not long that the couple broke off.ââ¬Å"Bundy's lack of confidence and tendency toward manipulation had ruined the relationshipâ⬠(ââ¬Å"Predator; Ted Bundyâ⬠BEGINNINGS). Many of his victims are said to resemble Brooks who had a major impact on him. Bundy returned to his birthplace in 1969 and discovered the facts about his mother. He returned to the University of Washington. Another woman Liz Kendall entered his life and filled it with love. He had everything in his life by 1973- a degree in psychology, a loving partner and an impressive job with the Washington State Republican Party (ââ¬Å"Predator; Ted Bundyâ⬠BEGINNINGS).Bundy became a brutal murderer by the end of 1973. He killed a number of girls in Washington. His first victim was 15 year old Kathy Devine. He abducted her on November 25, 1973 in a gre en pick-up and her body was recovered on December 6. Joni Lenz, his next victim, however was not killed though she suffered from brain damage and internal organ injuries. Lynda Ann Healy was abducted from her home on February 1, 1974 and never seen again. His next victims were Donna Manson, Susan Rancourt, Kathy Parks, Brenda Ball, Georgean Hawkins, Brenda Baker.All girls aged 15-19 years and Bundy followed a similar course of events in killing them- abduction and killing. Bundy carried on his inhuman routine and killed Janice Ott and Denise Naslund on July 14 at Lake Sammamish State Park. This time police could figure out a sketch and name Ted (though suspected to be unreal at first) of the criminal with the help of the people around (ââ¬Å"Predator; Ted Bundyâ⬠SERIAL KILLER IN WASHINGTON). Bundy went to Utah after killing about 11 young girls in Washington. He carried on his fury in Utah and claimed his first victim 16 year old Nancy Wilcox on October 2, 1974.Bundy raped, h it and murdered Melissa Smith and Laurie Aimee both 17 year old teenagers later in the month. He then tried to kidnap Carol DeRonch but she was lucky to escape. He was however successful in abducting another girl, Debbie Kent, later in the day who was not lucky like DeRonch. His next victims were Caryn Campbell, Julie Cunningham, Denise Oliverson, Melanie Cooley, Lynette Culver, Susan Curtis, Shelley Robertson, Nancy Baird and Debbie Smith. Most of the girlsââ¬â¢ bodies were not recovered and those recovered were mostly nude and severely injured (ââ¬Å"Predator; Ted Bundyâ⬠BUNDY GOES TO UTAH).Bob Haywood, Utah Highway Patrol Officer, suspected a VW in Granger, Utah and tried to have a look into it. The driver drove the vehicle away but was caught soon by Haywood. Driver was identified as Ted Bundy and officer found several doubtful things in his car like burglary tools, a mask made of panty hose, an icepick, and handcuffs. Bundy had come under police scanner and they tried to figure out his link with the abduction of DeRonch. DeRonch could not identify Bundy but a teacher at school from where Kent disappeared identified him. He was held in relation to DeRonch attack.Deronch later identified him and he was sent to jail for 1-15 years. He was then tried for the murder of Caryn Campbell. Police found her hair in Bundyââ¬â¢s VW. Bundy was defending himself in the case and while on the visit to the courthouse law library on June 7, 1977 escaped jumping from a two storey window. He was arrested again after 6 days of freedom. ââ¬Å"On December 30, 1977, he hacked his way through an old welded light fixture in his cell ceiling and crawled through to a deputies living quarters, put on some civilian clothes and walked out.He made his way to Vail, Colorado, took a bus to Denver, and boarded a plane to Chicago. â⬠He finally fled to Florida (ââ¬Å"Predator; Ted Bundyâ⬠ARRESTâ⬠¦ AND ESCAPE). Bundy could not resist his killer temptation for lon g and attacked 4 girls on the night of January 14, 1978- Lisa Levy, Margaret Bowman, Karen Chandler, and Kathy Kleiner. Two of them were killed and other two survived. He also claimed the life of Cheryl Thomas the same night. All of the girls were ruthlessly beaten, raped and strangled. He abducted and killed another girl Kimberly Ann Leach on February 9 Feb 2008.He stole VW again to escape but was soon arrested after some struggle and attempted fleeing (ââ¬Å"Predator; Ted Bundyâ⬠ON THE RUN IN FLORIDA). Bundy was tried for the murders in Florida and convicted on July 23. ââ¬Å"the bite marks on Levy's buttock and Nita Neary's eyewitness identification were too much to be overcome. Five days late the penalty phase began. Character witnesses were called by both sides including Mary Louise Bundy for the defenseâ⬠(ââ¬Å"Predator; Ted Bundyâ⬠LAST DAYS). On July 31 the verdict of death sentence came Bundyââ¬â¢s way.The state of Florida tried Bundy for Leachââ¬â ¢s murder and he was again convicted and sentenced to death. Bundy married Carol Ann Boone in the court who later gave birth to his daughter in October 1982. Bundy never admitted defeat and tried till end to escape his death sentence. Bundyââ¬â¢s death sentence was executed on January 24, 1989. He was electrocuted and declared dead at 7:16 am (ââ¬Å"Predator; Ted Bundyâ⬠LAST DAYS). Fig. 1 (ââ¬Å"Predator; Ted Bundyâ⬠LAST DAYS). Works Cited ââ¬Å"Predator; Ted Bundy. â⬠tedbundy. 150m. com. 22 July, 2008
Management Key Concepts
Organizational behavior is defined as a study aimed at revealing how individuals, people and groups are interacting. Simply saying, organizational behavior is norms and standards how employees should behave in work environment. Organizational behavior interprets people-organization relations within organization and social system as a whole. The purpose of organizational behavior is claimed to create more comfortable relations between employees and simply individuals by means of achieving organizational, human and social objectives.Organizations operate within the four models of behavior: autocratic, custodial, supportive, and collegial. Organizational behavior suggests that the potential of employees should be appreciated; organizational structure, people and technology work together; human problems should be encountered in work; problems are identified and corrective measures are taken. Organizational behavior is primary related to a workplace as it encompasses human behavior, human interactions, teams and leadership.(Damours, p.115)Organizational culture is defined as attitudes, beliefs, values and experiences within particular organization, firm or company. Organizational culture is a set of specific norms and values which must be shared by all employees, mangers and board of directors and which identify the way people should interact with each other and outside organization. Organization develops its own values stating how employees are expected to achieve the desired objectives and goals. Moreover, organizational culture prescribes norms of behavior in particular situations.The primary task is to integrate individuals into effective team which is able to adapt to external and internal environment of organization. Culture is ââ¬Å"an active living phenomenon through which people jointly create and recreate the worlds in which they liveâ⬠. (Damours, p.122) Elements of organizational culture are stated values, customs and rituals, comfortable climate, e tc. Organizational culture is related to a workplace as it sets norms and values of behavior and means of achieving organizational values.Diversity at workplace is defined as differences and similarities among organizational personnel. Diversity embraces, gender, age, cultural and ethnic background, physical abilities, religion, sexual orientation, etc. Nowadays diversity is essential concern in business world as companies are trying to retain the best employees not considering their age, gender or ethnicity.Workforce diversity means that organization develops practices and policies in order to include people who are considered to be different from what is considered normal. Diversity contributes social and corporate responsibility of organization as it gives an opportunity for everyone to earn living and to accomplish their dreams. Diversity also helps to turn tax users into effective tax players fostering in such a way economic development of the country. Diversity gives organizat ions advantage over companies which fully ignore diversity at workplace. (Damours, 134-137)Communication is defined as a means which convey verbal meanings creating shared understanding. Communication process requires interpersonal and intrapersonal processing, speaking, observing, listening, evaluating and analyzing. Communication process is related to a workplace is people are constantly interacting with other, arguing and debating. Future success of a person mainly depends on his ability to use language and to communicate effectively.Understanding how to communicate effectively will promote creating smoothly working project teams; responding to customers, clients, and markets; living and working in a culturally diverse world. Effective communication is playing nowadays one of the most important roles in organizations and companies, because it is the only source of mutual understanding among employees and customers, directors and suppliers, etc. Nevertheless learning how to commun icate effective isnââ¬â¢t limited only to one organization, because, for example, learning cross-cultural communication suggests how cultural traditions and patterns are understood and how cultural values may affect the process of communication. Learning effective communication is nowadays necessity. (Managing Communication, p.2)Business ethics is defined as a code of accepted norms, beliefs and values in business world. In other words, business ethics is a standard of human behavior that offers how to act in particular situations in professional life. Ethics incorporates norms of conventional morality to distinguish ââ¬Ëwrongââ¬â¢ behavior from ââ¬Ërightââ¬â¢ behavior. Generally, ethical norms suggest honesty, truthfulness, fairness, integrity, justice and respect for others. Ethical norms and values play important role in maintaining harmony and stability in social life as ethics suggests proper ways of human-human interactions.Ethics recognizes human needs and aspi rations, as well as cooperative efforts, fairness and truthfulness. Ethics contributes social stability and ensures balance in all spheres of life and business.à For example, in financial sphere ethical violations are associated with stakeholder interest, insider trading, investment management and camping financing. Business ethics is related to a workplace as it sets standards of behaviour within organization. (Damours, 205-209)Change management is defined as distinctly specifying and implementing procedures and methods to deal with changes in the organizational environment for the overall prosperity of the business. The term ââ¬Å"change managementâ⬠is mostly used to define ââ¬Å"organizational change management or the management of change triggered in organizations or industriesâ⬠. (Change Management, 2006) Change management is thus the overall process of grooming a planned approach to change in an organization.Due to globalization processes taken place in modern society change management has to be universal requirement. Change management deals primarily with the human aspect, because humans and their psychology are the most subjected to changes. There are two types of change management: reactive and proactive. Management is considered reactive when the change comes from external source. Management is considered proactive when change comes from either internal change aimed at achieving organizational objectives and goals. Change management is related to a workplace as changes are inevitable and mainly employees resist them.ReferencesManaging Communications. (1996, August). Peace Watch, 2, 5, 1-2.Coping With Change. (2006). Coping with Change: Public Sector Employees. The Paper Store Inc. Retrieved March 30, 2008, from http://search.1millionpapers.com/cgi-bin/query?mss=1millionpapers&q=Change+Public&source=googleppcDamours, Stephen. (1992). Management Analysis in Public Organizations: History, Concepts and Techniques. Westport: Quorum.
Friday, August 16, 2019
Importance Of Chemistry Essay
Our entire universe is made up of matter which is constantly changing forms and evolving into other forms of energy. We use chemistry from the beginning of the day till its end, the toothpaste we use to brush our teeth is made of abrasives, fluoride, and detergents. These common household items contain batteries, which make them very chemically dependent. The purification our water by process of chlorination, dental cleanliness from toothpaste, sterilization, the control and the cure of disease are all a part of daily living discovered and created by chemistry. These discoveries make up a major component for the protection of our health and hygiene and are vital for the future existence of mankind. Chemistry is the branch of science that deals with the identification of the substances of which matter is composed, of their properties and the ways in which they interact, combine, and change. The use of these processes to form new substances, understanding the importance of chemistry is essential to know the importance of chemistry in everyday life. Everything on earth is made up of chemicals you, your food, and the products you use in life such as soaps, perfumes etc. all are made up of chemicals. Life without chemicals is impossible. A number of changes that you observe are the result of chemical reactions like the rain, the change in the color of leaves, cleaning of any particular material in your house, all involve chemicals. You need to understand certain chemical reactions. When does your packet food expire? What disinfectants and repellents you should use to keep your house hygienic and healthy and so on. A guest (Jonathan Liu) stated ââ¬Å"I never knew that chemistry is applied to so many things in everyday life! I knew that there would be a lot of math involved and just thought that we would be studying science more in depth. Now that Iââ¬â¢ve read this, I am more interested in studying chemistry at schoolâ⬠. Chemistry is important because it helps in exploring things which are unknown to mankind. Chemistry is important because it explains the future behavior of plants and animals and all of us use chemicals directly or indirectly in our daily lives. We are us ing chemistry when we cook, when we wash, when we eat, when we get ready, when we play, when we think, as aà matter of fact, we always use chemistry. Increasing the knowledge in chemistry is important if we want to improve our lifestyle on the daily. Chemistry is also important for the environment. When you are aware of the harming effects of the gases like carbon dioxide and methane as a result of the greenhouse effect, you can easily carve out solutions for the reduction of effects on the environment. If you go with a minimum knowledge of the importance use of chemistry you can help in saving the environment to a large extent. Though chemistry is thought as complicated and a boring science subject, chemistry is responsible in explaining the world you live in, apart from explaining cooking, cleaning, medicine and environment issues; chemistry is in essence the study of everything. Understanding the basic chemistry is essential for all, but specializing in chemistry, making a career out of the subject is also interesting. Plenty of chemistry related high paying jobs are available today. All the medical students, physicists, geologists, nutritionists study chemistry. That the importance of chemistry will increase day by day and it is essential to understand the basic chemistry to properly conduct the activities of the daily life.
Thursday, August 15, 2019
Camels – Adaptations to Extreme Environments
The Camel: How it has Adapted Organisms exist in many environments, some are arid, some flooded, some are basic, some acidic, some are hot, and some are cool. Regardless of the conditions, animals have adapted themselves to survive in their surroundings. One of the main ecosystems that is classed as an extreme environment is the desert ecosystem. A classic example of a desert is the Sahara. Aridity is a characteristic shared amongst all deserts. As reported by Smith (2013), the reason most deserts are arid is because of uneven levels of evapotranspiration and precipitation.Aswel as adapting to very dry conditions, organisms in a desert ecosystem have also adapted to hot temperatures. The temperatures range from as high as 40oC during the day to as low as below freezing during the night due to lack of cloud cover. Evolution: As wrote by Long & Savage (1986) in the late Eocene period and to the end of Miocene, camels evolved and diversified solely in North America. During this time the y were similar in size to rabbits, and their feet contained four toes. In Oligocene times, camels were similar in size to goats, with the lost of the lateral toes, with the remaining digits showing signs of evolution.During the Miocene time, camels adapted a new of walking, it is know as pacing gait. This is where the camel moved both left or both right legs in one movement, instead of opposite legs. During this time camels feet produced pad like structures, which helped them from sinking in soft terrain i. e sand. Then they arrived in South America during the Plio-Pleistocene times. This is where they diversified into 2 more genus under Camelinae family of the more known species, llama and alpaca, and 2 less known, guanaco and vicuna.As this happened, they crossed into the ââ¬ËOld World', where they then spread across Europe, Asia and Africa, this is where they branched out into the ââ¬Ësingle humped' Camelus Dromedarius, and ââ¬Ëdouble humped' Camelus Bactrianus. Characte ristics of Camelus Dromedarius: An animal that has adapted well to this environment is the Camelus Dromedarius. Wilson (1984) as cited by Kohler-Rollefson (1991) describes the features of the camels body. Dromedary camels have a shoulder height of at least 1. 8m, with the hump extending a further 20cm. They can weights upwards of 400kg.Dromedary camels have a long upward curved neck, with a single hump on it's back. This is all supported by their long, strong legs and wide webbed feet. Body Temperature / Water Conservation: In the desert temperatures fluctuate. On average, a dehydrated camels body temperature fluctuates by up to 8oC in a single day, it can increase from an average of 34-36 to 42oC. There is less of a fluctuation if the camel is hydrated (2oC). If camels did not tolerate this fluctuation in temperature and instead tried to keep the body cool, up to 5 litres of water could be lost due to sweating.This allows the camel to conserve water and energy. The camel then takes advantage of the cool evenings to dissipate heat, with no energy cost. Camels, like all other mammals do sweat to maintain an acceptable temperature, however, it is not continuous. (Mares, 1999). Composition of Camel's Hump: Mares (1999), reports that a common misconception associated with camels is that their hump is composed of water, this has been disproven, and instead replaced with the fact this it is fat. Another common misconception associated with a camels hump is that water is produced by the oxidation of fat.This is has been proven to be false, or at least, partially untrue as the oxidation of fat requires an increased oxygen intake, this results in an increased rate of breathing, which increases the amount of water lost as vapour from the lungs. This nullifies the effect of the metabolized fat, yielding no net gain in water. As previously discussed, camels have adapted to keep their bodies cool, without sacrificing water. Another adaptation that is seen with camels is th at their main fat store is their hump. A camel does not store fat on other parts of their body.This is because the fat would act as insulation and reduce the amount of heat lost at night, at a time when the temperature is cooler, and most heat dissipation occurs. Excretion in Camels: Richards (1973) as cited by Mukasa-Mugerwa (1981) explores the fact that camels are able to produce urine that contains twice as much salt as sea water. It was noted by Mukasa-Mugerwa (1981) that the Kidney is composed of Henle loops of varying lengths. Camels are seen to contain a lot of longer loops than any other species, so there is a bigger potential for water to be reabsorbed, and the urine becoming concentrated.This coincides with the camels ability to facilitate water and plants with a salt content higher than normal. Schmidt-Nielsen (1964) as cited by Mukasa-Mugerwa (1981) compared the volume of urine excreted by the camel while hydrated and while dehydrated. The camel while hydrated, excreted up to 4 litres of urine in a single day, compared to the when it was dehydrated, where it excreted up to half a litre of urine. It was also noted that the camel produced faeces, almost completely absent of water.It was composed of a large number of pellets approximately 3cm in length. As they lacked water, they were light to lift, with a shiny appearance. Conclusion: Camels like animals in other extreme environments have adapted well to suit their environment of temperature fluctuations and a lack of water. Camels have evolved in such a way that moving has been made easier, they do not sink into the sand, so they do not expend more energy than is needed. The camels adaptation to a large fluctuation in body temperature allows water to be conserved as it is not lost as sweat.The camels hump proves as an invaluable energy source when food sources are scarce, as is common in desert ecosystems, the fat is metabolized and used as energy. It's all these adaptations that allows the camel to live in such an extreme environment. The absence of one or more of these adaptations would lead to the inability of camels to survive in this environment. In this day and age global warming is increasing the average temperature of the earths atmosphere, if there was a significant increase, could these organism handle an increased temperature luctuation? References: Jeremy M. B. Smith desert 2013. Encyclop? dia Britannica Online. Retrieved 01 March, 2013, from http://www. britannica. com/EBchecked/topic/158992/desert Kohler-Rollefson, Ilse U, (1991). Camelus Dromedarius. Mammalian Species. 375 (1-8), pp. 1-4 Mares, Michael A. , (1999). Encyclopaedia of Deserts. 1st ed. Oklahoma: University of Oklahoma Press. Richards, S. A. , 1973. Adaptation to heat. In Temperature regulation. London, Wykeham Publications, pp. 191-197 as cited by Mukasa-Mugerwa, E. , (1981).The Camel (Camelus Dromedarius): A Bibliographical Review. 1st ed. Ethiopia: International Livestock Centre of Africa Savage R . J. G & Long, M. R. , (1986). Mammal Evolution. 1st ed. United Kingdom: Facts on File & The British Museum. Schmidt-Nielsen, K. 1964. The Camel. In Desert Animals: physiological problems of heat and water. Oxford, Clarendon press, 277 pp as cited by Mukasa-Mugerwa, E. , (1981). The Camel (Camelus Dromedarius): A Bibliographical Review. 1st ed. Ethiopia: International Livestock Centre of Africa
Wednesday, August 14, 2019
Bar coding
A taxonomic method that identifies the species of an organism by using a small genetic marker in the mitochondrial DNA of the organism is DNA bar coding. The mitochondrion of most eukaryotes contains the mitochondrial DNA, and the rate of mutations is faster that are responsible for a significant variation in the DNA sequences of the mitochondria between the species. The identification of the species via DNA bar coding relies entirely upon the short fragment of mitochondrial DNA.Recently interest is increasing to use a short sequence of tags namely bar codes for identifying and documentation of the species. (Blaxter,2003, Hebert and Gregory,2005). The standard sequence of the DNA used as a bar code is the mitochondrial gene cytochrome c oxidase I or COI. A number of studies show that utilizing barcode gives accuracy in the classification of the species. (Kerret. et. al, 2007). The chitons live near the edge of the ocean they are mollusks.The chitons of the north pacific region are di verse comprising around 80 species, and all these species belong to the genus Mopalia. (Eernisse). The present paper discusses on identification of the species of the genus Mopalia from the extremely diverse community of chitons of the north pacific region using the DNA bar coding. DNA bar coding: To characterize the species of the organisms a novel technique exists termed ââ¬Å"DNA bar codingâ⬠, and the technique uses a short sequence of DNA on the standard position of the genome.Obtaining the DNA sequences is quick as they comprise a very short sequence in comparison with the whole genome. The cytochrome oxidase subunit 1 of the mitochondrial region is the standard bar code region that contains a length of 648 nucleotide base pairs. There is a variation of the DNA bar codes among the individuals of the same species, but it is little. The effectiveness of the DNA bar code is directly dependent on the minor variation within the species, which is smaller than the differences be tween the species.(Consortium). Genus Mopalia: Fauna and flora of the north pacific region is diverse with long coastline and cool temperatures. Many species of the chitons play an important role in the ecology of the rocky shorelines. The Mopalia group has its recent origin with an interesting morphological similarity that attracts case study for DNA bar coding. DNA bar coding using the genus Mopalia: Up to now focus of the studies on DNA bar coding was mainly with the collection of the species within the geographical areas. (Hebert et. al,2003a).Studies in DNA bar coding with reference to the monophyletic groups is less leaving the ambiguity whether this technique is useful in distinguishing the closely related species. (Moritz and Cicero,1994)The present study uses the genus Mopalia, a challenging case study for the DNA bar coding methodology. The genus Mopalia is a recently originated; monophyletic that has a wider geographical occurrence. (Kelly et. al, 2007). Kelly et. al stud ied 19 species of the genus Mopalia, and six closely related species that out group the 131 individuals of the total of the 25 species used.The character-based assessment referred to as the characteristic attribute organizations system is fast, that avoids the false-positive data and it also retains the information on evolution that is in the character-state data. (Desalle et. al, 2005). This approach gives a new perspective to the methods of DNA bar coding. This kind of information is more like a traditional taxonomy. (Desalle et. al, 2005). The CAOS method or the character based method gives clear information, and getting the information is faster, and there is accuracy in the identification of the query sequences of the given data set.The CAOS method is sensitive that distinguishes the taxa of the monophyletic group of the closely related species with a very short sequence of the mitochondrial DNA. The advantage in this method is that it preserves the evolutionary information in the form of character data that avoids the false-positive identification of the query sequences. The number of diagnostics that supports the placement of a query sequence in a clade also used as a confidence value. The bar coding group has no relevance with the CAOS method; this is because the diagnostic character distinguishes monophyletic groups.While distinguishing the monophyletic groups, reference is not a requirement about the relative degree of divergence within and between the taxa. The additional advantage with the CAOS approach is its use as a means of cataloguing the diagnostic molecular characteristics used to distinguish the species. These diagnostics complement the traditional taxonomy. (De Salle et. al, 2005). Even though the description of the species depends on the multiple lines of evidence, the CAOS approach is a good tool to identify significant intraspecific divergences.Additional evidence that includes morphological, geographical, ecological, and behavioral tha t is diagnostic for the new taxon. The CAOS method preserves the character state information, reports diagnostic differences for each node. This method helps the taxonomists to identify and describe divergent organisms that require further scrutiny. (Blaxter,2004). In this method threshold values for the species level are not necessary, which addresses the critical issues in other methods. (Meyer and Paulay,2005). This method of DNA bar coding corrects some pitfalls of the distance based approaches.The inclusion of the evolutionary and taxonomic information in this method gives a context in which there is interpretation of the sequence data and gives a real promise to the DNA bar coding. The CAOS method gives a way to the researchersââ¬â¢ confrontation to identify and make the sense of diversity. Conclusions: The distance based methods for DNA bar coding lack the accuracy that largely depend on the degree of disparity between the intra and site-specific variation that is the bar coding gap. (Meyer and Paulay,2005).Insufficiency in sampling the taxon will increase the disparity that increased the apparent accuracy. CAOS method of DNA bar coding addresses the problems associated with the distance based methods of bar coding. References: Blaxter, M. (2003). Molecular systematicsââ¬âcounting angels with DNA, Nature, 2003, vol. 421, pp. 122ââ¬â124 Hebert, P. D. N. & Gregory, T. R. (2005),The promise of DNA bar coding for taxonomy. System Biology (54), pp. 852ââ¬â859. DNA bar coding, consortium for the bar code of life. Available on http://barcoding. si. edu/, retrieved on 30 October 2007.
Subscribe to:
Posts (Atom)